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Bayer AG hmec-t53 cells
Hmec T53 Cells, supplied by Bayer AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmec-t53+cells/hmec+t53+cells/pm23024001-34-8-14
Average 90 stars, based on 1 article reviews
hmec-t53 cells - by Bioz Stars, 2026-09
90/100 stars

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Article Snippet: Inhibition of histone deacetylase (HDAC) activity by small molecules has been shown to provide a therapeutic benefit to patients with diverse diseases including malignancies.. The HDAC family is divided into classes I, IIa/b and IV, which together comprise 11 zinc-dependent enzymes, as well as into class III, which encompasses the 7 NADþ-dependent sirtuins.. HDACs deacetylate both nuclear histone and nonhistone proteins such as transcription factors, and thereby play important roles in the regulation of numerous cellular processes.



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PromoCell hmec t53 cells
(A) Array Northern analysis of DNMT3B mRNA expression on Affymetrix HGU133Plus2.0 arrays in tumor (T) tissue samples compared to normal (N) tissues of the same organ. n indicates the number of analyzed samples for the specified tissue type. Data are shown as mean±SD; *P<0.05 relative to normal tissue (N), **P<0.01 relative to normal tissue (N), ***P<0.001 relative to normal tissue (N), determined by student's t-test. (B) Quantitative real-time PCR analysis of DNMT3B mRNA expression in colon cancer cell lines and normal cells <t>(HMEC-T53,</t> WI-38). Expression values are means of triplicates and were calculated relative to GAPDH expression. Error bars represent standard errors. (C) Immunoblot of DNMT3B protein expression in lysates from various colon cancer cell lines and normal cells (HMEC-T53, WI-38). ß-actin was used as a loading control.
Hmec T53 Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmec-t53+cells/Mammary+Epithelial+Cell+Growth+Medium/pmc03341356-38-19-29
Average 94 stars, based on 1 article reviews
hmec t53 cells - by Bioz Stars, 2026-09
94/100 stars
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90
Bayer AG hmec-t53 cells
(A) Array Northern analysis of DNMT3B mRNA expression on Affymetrix HGU133Plus2.0 arrays in tumor (T) tissue samples compared to normal (N) tissues of the same organ. n indicates the number of analyzed samples for the specified tissue type. Data are shown as mean±SD; *P<0.05 relative to normal tissue (N), **P<0.01 relative to normal tissue (N), ***P<0.001 relative to normal tissue (N), determined by student's t-test. (B) Quantitative real-time PCR analysis of DNMT3B mRNA expression in colon cancer cell lines and normal cells <t>(HMEC-T53,</t> WI-38). Expression values are means of triplicates and were calculated relative to GAPDH expression. Error bars represent standard errors. (C) Immunoblot of DNMT3B protein expression in lysates from various colon cancer cell lines and normal cells (HMEC-T53, WI-38). ß-actin was used as a loading control.
Hmec T53 Cells, supplied by Bayer AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hmec-t53+cells/hmec+t53+cells/pm23024001-34-8-14
Average 90 stars, based on 1 article reviews
hmec-t53 cells - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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(A) Array Northern analysis of DNMT3B mRNA expression on Affymetrix HGU133Plus2.0 arrays in tumor (T) tissue samples compared to normal (N) tissues of the same organ. n indicates the number of analyzed samples for the specified tissue type. Data are shown as mean±SD; *P<0.05 relative to normal tissue (N), **P<0.01 relative to normal tissue (N), ***P<0.001 relative to normal tissue (N), determined by student's t-test. (B) Quantitative real-time PCR analysis of DNMT3B mRNA expression in colon cancer cell lines and normal cells (HMEC-T53, WI-38). Expression values are means of triplicates and were calculated relative to GAPDH expression. Error bars represent standard errors. (C) Immunoblot of DNMT3B protein expression in lysates from various colon cancer cell lines and normal cells (HMEC-T53, WI-38). ß-actin was used as a loading control.

Journal: PLoS ONE

Article Title: Antiproliferative Effects of DNA Methyltransferase 3B Depletion Are Not Associated with DNA Demethylation

doi: 10.1371/journal.pone.0036125

Figure Lengend Snippet: (A) Array Northern analysis of DNMT3B mRNA expression on Affymetrix HGU133Plus2.0 arrays in tumor (T) tissue samples compared to normal (N) tissues of the same organ. n indicates the number of analyzed samples for the specified tissue type. Data are shown as mean±SD; *P<0.05 relative to normal tissue (N), **P<0.01 relative to normal tissue (N), ***P<0.001 relative to normal tissue (N), determined by student's t-test. (B) Quantitative real-time PCR analysis of DNMT3B mRNA expression in colon cancer cell lines and normal cells (HMEC-T53, WI-38). Expression values are means of triplicates and were calculated relative to GAPDH expression. Error bars represent standard errors. (C) Immunoblot of DNMT3B protein expression in lysates from various colon cancer cell lines and normal cells (HMEC-T53, WI-38). ß-actin was used as a loading control.

Article Snippet: WI-38 cells were maintained in MEM Earle's medium supplemented with L-glutamine (Biochrom) and 10% fetal calf serum (PAA), and HMEC-T53 cells were grown in Mammary Epithelial Cell Growth Medium (Promocell: C-21010) and SupplementMix (Promocell: C-39115).

Techniques: Northern Blot, Expressing, Real-time Polymerase Chain Reaction, Western Blot